https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Reactive oxygen species form part of a regulatory pathway initiating trans-differentiation of epidermal transfer cells in Vicia faba cotyledons https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27813 Wed 11 Apr 2018 12:10:22 AEST ]]> Ethylene and hydrogen peroxide regulate formation of a sterol-enriched domain essential for wall labyrinth assembly in transfer cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47611 Vicia faba cotyledons. When these cotyledons are placed in culture, their adaxial epidermal cells trans-differentiate to a TC phenotype regulated by auxin, ethylene, extracellular hydrogen peroxide (apoH2O2), and cytosolic Ca2+ ([Ca2+]cyt) arranged in series. Staining cultured cotyledons with the sterol-specific dye, Filipin III, detected a polarized sterol-enriched domain in the plasma membrane of their trans-differentiating epidermal transfer cells (ETCs). Ethylene activated sterol biosynthesis while extracellular apoH2O2 directed sterol-enriched vesicles to fuse with the outer periclinal region of the ETC plasma membrane. The sterol-enriched domain was essential for generating the [Ca2+]cyt signal and orchestrating construction of both the uniform wall layer and wall ingrowth papillae. A model is presented outlining how the sterol-enriched plasma membrane domain forms and functions to regulate wall labyrinth assembly.]]> Tue 24 Jan 2023 11:28:41 AEDT ]]> Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6985 Sat 24 Mar 2018 08:37:50 AEDT ]]>